All kick-off times in GMT.
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- Wed, Nov 21, 2012
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| FT | Central Norte | 3 - 2 | Altos Hornos Zapla |
| FT | Libertad (S) | 0 - 1 | Ben Hur |
| FT | Alumni | 0 - 2 | Sp. Rivadavia (Venado Tuerto) |
| FT | Tiro Federal | (3)1 - 1(4) | Dep. Mandiyú |
| FT | San Jorge (Tucumán) | (3)1 - 1(2) | Sportivo Patria |
| FT | Def. de Belgrano (VR) | 3 - 0 | Once Tigres |
| FT | Rivadavia (L) | 3 - 5 | Agropecuario Argentino |
| FT | Santamarina | 1 - 0 | Grupo Universitario |
| FT | Alvarado | 3 - 2 | Sol de Mayo (Viedma) |
| FT | Guillermo Brown | 1 - 0 | C.A.I. |
| FT | Platense | 1 - 0 | Liniers |
| FT | Deportivo Armenio | (3)1 - 1(1) | El Porvenir |
| FT | San Telmo | (2)1 - 1(3) | Midland |
| FT | Chacarita Juniors | 4 - 0 | Claypole |
| FT | Flandria | (4)0 - 0(5) | Excursionistas |
| FT | Brown de Adrogué | 1 - 0 | L.N. Alem |
| FT | Tristán Suárez | 4 - 0 | Laferrere |
| Group A | |||
|---|---|---|---|
| FT | Deportivo Pasto | 1 - 0 | Atlético Junior |
| Group B | |||
| FT | Itagüí | 0 - 1 | La Equidad |
| FT | Atlético Nacional | 0 - 1 | Independiente Medellín |
| FT | Rubio Ñú | 2 - 7 | Libertad |
| FT | Sol de América | 2 - 0 | Nacional |
| FT | Sp. Carapeguá | 5 - 1 | Sportivo Luqueño |
| FT | Guaraní | 1 - 0 | Cerro Porteño de Franco |
| FT | Olimpia | 2 - 2 | Tacuary |
| FT | El Nacional | 0 - 2 | Independiente Terán |
| FT | LDU Quito | 3 - 0 | Deportivo Quito |
| FT | Barcelona | 0 - 0 | Manta F.C. |
| FT | Club Deportivo Cuenca | 0 - 0 | Macará |
| FT | Liga de Loja | 2 - 2 | Emelec |
| FT | Tec. Universitario | 2 - 1 | Olmedo |