All kick-off times in GMT.
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- Sat, Jan 19, 2013
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| FT | Northampton Town | 1 - 0 | Burton Albion |
| FT | Wycombe Wanderers | 1 - 0 | Dagenham & Redbridge |
| FT | York City | 0 - 0 | Aldershot Town |
| FT | AFC Telford United | 0 - 0 | Alfreton Town |
| FT | Macclesfield Town | 1 - 0 | Kidderminster Harriers |
| FT | Newport County | 0 - 2 | Barrow |
| FT | Southport | 1 - 2 | Mansfield Town |
| FT | Stockport County | 2 - 1 | Forest Green Rovers |
| FT | St Mirren | 1 - 4 | Ross County |
| FT | Celtic | 4 - 1 | Heart of Midlothian |
| FT | Hibernian | 1 - 1 | Dundee |
| FT | Inverness Caledonian Thistle | 3 - 0 | Aberdeen |
| FT | Kilmarnock | 2 - 3 | Dundee United |
| FT | Airdrie United | 1 - 2 | Dumbarton |
| FT | Greenock Morton | 2 - 1 | Livingston |
| FT | Partick Thistle | 4 - 1 | Falkirk |
| FT | Raith Rovers | 0 - 2 | Hamilton Academical |
| FT | Granada | 2 - 0 | Rayo Vallecano |
| FT | Real Sociedad | 3 - 2 | Barcelona |
| FT | Getafe | 1 - 1 | Sevilla FC |
| FT | Malaga | 1 - 1 | Celta Vigo |
| FT | CD Sabadell | 4 - 1 | Girona |
| FT | Guadalajara | 3 - 0 | AD Alcorcon |
| FT | Las Palmas | 1 - 0 | Racing Santander |
| FT | Real Madrid Castilla | 5 - 0 | Villarreal |
| FT | Sochaux | 1 - 0 | Stade de Reims |
| FT | Marseille | 3 - 2 | Montpellier |
| FT | AC Ajaccio | 1 - 1 | Valenciennes |
| FT | Brest | 0 - 1 | St Etienne |
| FT | Lorient | 3 - 2 | Troyes |
| FT | Toulouse | 2 - 1 | AS Nancy Lorraine |
| FT | Heerenveen | 0 - 1 | SC Heracles Almelo |
| FT | NAC Breda | 1 - 0 | VVV Venlo |
| FT | Twente Enschede | 0 - 0 | RKC Waalwijk |
| FT | AZ Alkmaar | 4 - 1 | Vitesse Arnhem |
| FT | KAA Gent | 0 - 0 | Standard Liege |
| FT | KV Kortrijk | 4 - 0 | Germinal Beerschot |
| FT | Lierse SK | 0 - 0 | Waasland-Beveren |
| FT | OH Leuven | 2 - 6 | KSC Lokeren |
| FT | Racing Genk | 3 - 1 | Royal Charleroi SC |
| FT | RAEC Bergen Mons | 2 - 3 | KV Mechelen |
| FT | Ballinamallard Utd | 3 - 1 | Glenavon |
| FT | Coleraine | 3 - 1 | Lisburn Distillery |
| FT | Crusaders | 5 - 1 | Ballymena |
| FT | Portadown | 1 - 3 | Cliftonville |
| FT | Internacional | 1 - 1 | Passo Fundo |
| FT | São José-POA | 1 - 0 | Pelotas |
| FT | Juventude | 1 - 1 | Lajeadense |
| FT | Central Coast Mariners | 0 - 0 | Newcastle Jets |
| FT | Perth Glory | 0 - 1 | Melbourne Victory |
| FT | Brisbane Roar | 1 - 2 | Western Sydney FC |
| FT | Universidad | 0 - 0 | Malacateco |
| FT | Comunicaciones | 1 - 0 | Petapa |
| FT | Juventud Escuintleca | 1 - 1 | Mictlan |