All kick-off times in GMT.
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- Sat, Jan 26, 2013
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| FT | Bristol City | 2 - 1 | Ipswich Town |
| FT | Burnley | 1 - 2 | Birmingham City |
| FT | Charlton Athletic | 1 - 2 | Sheffield Wednesday |
| FT | Nottingham Forest | 0 - 3 | Watford |
| FT | Wolverhampton Wanderers | 1 - 2 | Blackpool |
| FT | Cambridge United | 0 - 0 | Grimsby Town |
| FT | Lincoln City | 1 - 2 | Forest Green Rovers |
| FT | Nuneaton Town | 2 - 4 | Braintree Town |
| FT | Dumbarton | 3 - 1 | Hamilton Academical |
| FT | Dunfermline Athletic | 0 - 1 | Livingston |
| FT | Falkirk | 4 - 3 | Airdrie United |
| FT | Greenock Morton | 1 - 0 | Raith Rovers |
| FT | Partick Thistle | 2 - 1 | Cowdenbeath |
| FT | Celta Vigo | 1 - 1 | Real Sociedad |
| FT | Levante | 2 - 1 | Real Valladolid |
| FT | Real Zaragoza | 0 - 0 | Espanyol |
| FT | Deportivo La Coruña | 2 - 3 | Valencia |
| FT | Girona | 2 - 0 | Guadalajara |
| FT | Lugo | 3 - 1 | Las Palmas |
| FT | Murcia | 1 - 1 | Sporting Gijon |
| FT | Racing Santander | 1 - 3 | Cordoba |
| FT | Elche | 2 - 0 | Hercules |
| FT | Bari | 0 - 1 | Ascoli |
| FT | Cesena | 3 - 1 | Vicenza |
| FT | Empoli | 0 - 2 | Novara |
| FT | Grosseto | 3 - 1 | Cittadella |
| FT | Modena | 1 - 2 | Ternana |
| FT | Padova | 0 - 0 | Livorno |
| FT | Pro Vercelli | 0 - 0 | Reggina |
| FT | Spezia | 0 - 1 | Hellas Verona |
| FT | Varese | 1 - 2 | Lanciano |
| FT | Stade Rennes | 2 - 2 | Marseille |
| FT | AS Nancy Lorraine | 2 - 1 | Lorient |
| FT | Evian Thonon Gaillard | 1 - 1 | AC Ajaccio |
| FT | Montpellier | 2 - 0 | Sochaux |
| FT | Stade de Reims | 1 - 1 | Toulouse |
| FT | Troyes | 2 - 1 | Brest |
| FT | PEC Zwolle | 1 - 1 | Heerenveen |
| FT | ADO Den Haag | 2 - 2 | Roda JC Kerkrade |
| FT | RKC Waalwijk | 0 - 4 | NAC Breda |
| FT | SC Heracles Almelo | 1 - 5 | PSV Eindhoven |
| FT | Istanbul Buyuksehir BSK | 4 - 1 | Bursaspor |
| FT | Genclerbirligi | 1 - 0 | Akhisar Belediye |
| FT | Gaziantepspor | 1 - 2 | Fenerbahce |
| FT | KSC Lokeren | 0 - 2 | Anderlecht |
| FT | Germinal Beerschot | 0 - 0 | RAEC Bergen Mons |
| FT | KV Mechelen | 2 - 1 | Racing Genk |
| FT | Waasland-Beveren | 2 - 0 | OH Leuven |
| FT | Zulte-Waregem | 2 - 0 | Lierse SK |
| FT | Cruz Azul | 4 - 0 | Puebla |
| FT | Querétaro | 0 - 2 | U.A.N.L |
| FT | Monterrey | 3 - 2 | San Luis |
| FT | Tijuana | 2 - 0 | Pachuca |
| FT | Atlas | 2 - 1 | América |
| FT | LDU Quito | 2 - 1 | U. Católica |
| FT | Manta F.C. | 3 - 1 | Independiente Terán |
| FT | Deportivo Quevedo | 1 - 1 | Barcelona |
| FT | Western Sydney FC | 1 - 0 | Melbourne Heart |
| FT | Perth Glory | 0 - 1 | Brisbane Roar |
| FT | Wellington Phoenix FC | 1 - 1 | Newcastle Jets |
| FT | Marquense | 2 - 0 | Suchitepequez |
| FT | Malacateco | 2 - 0 | Heredia |
| FT | Petapa | 1 - 2 | Universidad |
| FT | Xelaju | 3 - 1 | Juventud Escuintleca |
| FT | Real Espana | 1 - 1 | Platense |
| FT | Olimpia | 2 - 1 | Atletico Choloma |