All kick-off times in GMT.
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- Sat, Feb 16, 2013
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| FT | Luton Town | 0 - 3 | Millwall |
| FT | Arsenal | 0 - 1 | Blackburn Rovers |
| FT | Milton Keynes Dons | 1 - 3 | Barnsley |
| FT | Oldham Athletic | 2 - 2 | Everton |
| FT | Celtic | 6 - 2 | Dundee United |
| FT | Heart of Midlothian | 0 - 3 | Kilmarnock |
| FT | Motherwell | 3 - 0 | Inverness Caledonian Thistle |
| FT | Ross County | 1 - 0 | St Johnstone |
| FT | St Mirren | 0 - 1 | Hibernian |
| FT | Airdrie United | 2 - 2 | Hamilton Academical |
| FT | Falkirk | 1 - 0 | Dunfermline Athletic |
| FT | Greenock Morton | 2 - 2 | Partick Thistle |
| FT | Livingston | 3 - 0 | Cowdenbeath |
| FT | Raith Rovers | 3 - 2 | Dumbarton |
| FT | Arbroath | 0 - 1 | Brechin City |
| FT | Ayr United | 5 - 2 | Albion Rovers |
| FT | Forfar Athletic | 0 - 1 | Alloa Athletic |
| FT | Stenhousemuir | 2 - 1 | East Fife |
| FT | Stranraer | 0 - 5 | Queen of the South |
| FT | Annan Athletic | 1 - 2 | East Stirlingshire |
| FT | Clyde | 1 - 4 | Rangers |
| FT | Montrose | 4 - 1 | Elgin City |
| FT | Peterhead | 0 - 0 | Stirling Albion |
| FT | Queen's Park | 2 - 1 | Berwick Rangers |
| FT | Getafe | 3 - 1 | Celta Vigo |
| FT | Malaga | 1 - 0 | Athletic Bilbao |
| FT | Granada | 1 - 2 | Barcelona |
| FT | Osasuna | 1 - 0 | Real Zaragoza |
| FT | Marseille | 1 - 0 | Valenciennes |
| FT | Bastia | 0 - 1 | Nice |
| FT | Brest | 1 - 1 | AC Ajaccio |
| FT | Lorient | 2 - 1 | Evian Thonon Gaillard |
| FT | Montpellier | 1 - 0 | AS Nancy Lorraine |
| FT | Toulouse | 2 - 2 | Troyes |
| FT | Twente Enschede | 1 - 1 | Willem II Tilburg |
| FT | NAC Breda | 1 - 1 | SC Heracles Almelo |
| FT | Roda JC Kerkrade | 2 - 2 | AZ Alkmaar |
| FT | PSV Eindhoven | 2 - 1 | FC Utrecht |
| FT | Cercle Brugge KSV | 0 - 1 | Standard Liege |
| FT | Lierse SK | 0 - 2 | KV Mechelen |
| FT | OH Leuven | 1 - 1 | Germinal Beerschot |
| FT | RAEC Bergen Mons | 0 - 2 | KAA Gent |
| FT | Zulte-Waregem | 2 - 0 | Waasland-Beveren |
| FT | SV Josko Ried | 3 - 0 | FC Wacker Tirol |
| FT | FC Trenkwalder Admira | 1 - 2 | FC Magna Wr. Neustadt |
| FT | SV Mattersburg | 0 - 0 | SK Sturm Graz |
| FT | Ballymena | 0 - 1 | Coleraine |
| FT | Donegal Celtic | 1 - 0 | Ballinamallard Utd |
| FT | Dungannon Swifts | 2 - 0 | Portadown |
| FT | Glentoran | 1 - 1 | Linfield |
| FT | Lisburn Distillery | 2 - 1 | Glenavon |
| FT | Godoy Cruz de Mendoza | 2 - 1 | Unión de Santa Fe |
| FT | San Lorenzo | 0 - 0 | Belgrano de Córdoba |
| FT | Colón de Santa Fe | 1 - 1 | San Martín de San Juan |
| FT | Vélez Sarsfield | 0 - 1 | Independiente |
| FT | América | 2 - 2 | Toluca |
| FT | Pachuca | 2 - 1 | Cruz Azul |
| FT | U.A.N.L | 1 - 1 | Santos |
| FT | León | 2 - 0 | San Luis |
| FT | Atlas | 2 - 1 | Monterrey |
| FT | Everton CD | 1 - 1 | Universidad de Chile |
| FT | Unión La Calera | 1 - 0 | Municipal Iquique |
| FT | Universidad Católica | 4 - 2 | Antofagasta |
| FT | Arica | 0 - 4 | Ñublense |
| FT | Patriotas F.C. | 1 - 3 | Cúcuta Deportivo |
| FT | Atlético Junior | 2 - 2 | Boyacá Chicó FC |
| FT | Millonarios | 1 - 0 | Envigado |
| FT | Atlético Nacional | 4 - 0 | Alianza Petrolera |
| FT | Melbourne Victory | 1 - 2 | Western Sydney FC |
| FT | Perth Glory | 2 - 1 | Central Coast Mariners |
| FT | Brisbane Roar | 2 - 0 | Wellington Phoenix FC |