All kick-off times in GMT.
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- Fri, Mar 1, 2013
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| FT | FC Union Berlin | 3 - 0 | FC Erzgebirge Aue |
| FT | SC Paderborn 07 | 0 - 1 | Energie Cottbus |
| FT | SSV Jahn Regensburg | 1 - 3 | SV Sandhausen |
| FT | Ajaccio GFCO | 0 - 1 | AS Monaco |
| FT | Arles | 2 - 1 | AJ Auxerre |
| FT | Dijon FCO | 1 - 1 | Niort |
| FT | FC Istres | 1 - 0 | Sedan |
| FT | Le Havre AC | 1 - 0 | Chateauroux |
| FT | Le Mans | 0 - 2 | Angers |
| FT | Stade Laval | 1 - 1 | Clermont Foot |
| FT | Tours | 1 - 1 | Caen |
| Canc | BV Veendam | OFF | FC Volendam |
| FT | FC Oss | 1 - 0 | FC Eindhoven |
| FT | Helmond Sport | 1 - 1 | Excelsior |
| FT | MVV Maastricht | 1 - 2 | FC Emmen VV |
| FT | SC Stormvogels Telstar | 1 - 3 | Go Ahead Eagles |
| FT | Sparta Rotterdam | 2 - 1 | De Graafschap |